Abstract | BACKGROUND: METHODS: A piglet model of extended cardiac arrest (12 min of untreated cardiac arrest and 8 min of CPR) was used to assess possible additional neuroprotective effects of MB when administered during CPR before mild therapeutic hypothermia induced 30 min after restoration of spontaneous circulation (ROSC). Three groups were compared: C group (n = 8) received standard CPR; PH group (n = 8) received standard CPR but 30 min after ROSC these piglets were cooled to 34°C; the PH+MB group (n = 8) received an MB infusion 1 min after commencement of CPR and the same cooling protocol as the PH group. Three hours later, the animals were killed. Immediately after death, the brains were harvested pending histological and immunohistological analysis. RESULTS: Circulatory variables were similar in the groups except that cardiac output was greater in the PH+MB group 2-3 h after ROSC. Cerebral cortical neuronal injury and blood-brain barrier disruption was greatest in the C group and least in the MB group. The neuroprotective effect of MB and hypothermia was significantly greater than that of delayed hypothermia alone. CONCLUSION:
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Authors | L Wiklund, F Zoerner, E Semenas, A Miclescu, S Basu, H S Sharma |
Journal | Acta anaesthesiologica Scandinavica
(Acta Anaesthesiol Scand)
Vol. 57
Issue 8
Pg. 1073-82
(Sep 2013)
ISSN: 1399-6576 [Electronic] England |
PMID | 23577658
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 The Acta Anaesthesiologica Scandinavica Foundation. Published by John Wiley & Sons Ltd. |
Chemical References |
- Biomarkers
- Neuroprotective Agents
- Methylene Blue
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Topics |
- Animals
- Biomarkers
- Blood Gas Analysis
- Blood Pressure
(physiology)
- Blood-Brain Barrier
(drug effects, physiology)
- Body Temperature
(physiology)
- Cardiac Output
(physiology)
- Cardiopulmonary Resuscitation
(methods)
- Cerebral Cortex
(pathology)
- Data Interpretation, Statistical
- Female
- Heart Arrest
(therapy)
- Hypothermia, Induced
- Immunohistochemistry
- Lipid Peroxidation
(drug effects)
- Male
- Methylene Blue
(pharmacology)
- Neuroprotective Agents
- Oxygen Consumption
(physiology)
- Survival
- Swine
- Ventricular Fibrillation
(physiopathology, therapy)
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