A mouse model for studying the clearance of hepatitis B virus in vivo using a luciferase reporter.
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| Abstract |
Hepatitis B virus(HBV) infection remains a global problem, despite the effectiveness of the Hepatitis B vaccine in preventing infection. The resolution of Hepatitis B virus infection has been believed to be attributable to virus-specific immunity. In vivo direct evaluation of anti-HBV immunity in the liver is currently not possible. We have developed a new assay system that detects HBV clearance in the liver after the hydrodynamic transfer of a reporter gene and over-length, linear HBV DNA into hepatocytes, followed by bioluminescence imaging of the reporter gene (Fluc). We employed bioluminescence detection of luciferase expression in HBV-infected hepatocytes to measure the Hepatitis B core antigen (HBcAg)-specific immune responses directed against these infected hepatocytes. Only HBcAg-immunized, but not mock-treated, animals decreased the amounts of luciferase expression, HBsAg and viral DNA from the liver at day 28 after hydrodynamic infection with over-length HBV DNA, indicating that control of luciferase expression correlates with viral clearance from infected hepatocytes.
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| Authors | Sheng-qiang Liang, Juan Du, Hu Yan, Qian-qian Zhou, Yong Zhou, Zhen-nan Yuan, Shao-duo Yan, Qiu-xia Fu, Xiao-hui Wang, Shuai-zheng Jia, Jian-chun Peng, Yang-gen Zhang, Lin-sheng Zhan |
| Journal | PloS one (PLoS One) Vol. 8 Issue 4 Pg. e60005 ( 2013) ISSN: 1932-6203 [Electronic] United States |
| PMID | 23577080 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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