Beta-catenin and survivin expression in keratocystic odontogenic tumor (KCOT). A comparative immunohistochemical study in primary, recurrent and nevoid basal cell carcinoma syndrome (NBCCS)-associated lesions.

Abstract

AIM:

To determine the epithelial expression of β-catenin and survivin in sporadic (primary, and recurrent) and nevoid basal cell carcinoma syndrome (NBCCS) keratocystic odontogenic tumour (KCOT) in order to assess activation of the β-catenin pathway and evidence of apoptotic inhibition, processes that may contribute to the known differences in their biological behaviour.

MATERIALS AND METHODS:

Sections from 40 cases of KCOT (19 sporadic/primary; 9 sporadic/recurrent and 12 NBCCS-associated) were immunohistochemically stained for β-catenin and survivin. The extent and intensity of immunoreactivity within the lining epithelium was assessed, using semi-quantitative scales, independently by two pathologists who were blinded to the clinical-pathological data. Data were analysed using Kruskal-Wallis test and, for pair-wise comparisons, Mann-Whitney test with Bonferroni correction.

RESULTS:

All cystic epithelial linings stained for β-catenin and survivin but there were differences in the pattern and intensity of staining among KCOT types. Sporadic primary KCOT showed weaker staining for β-catenin (P=0.0003) and survivin (P<0.0048) that was restricted to the basal and para-basal layers only, compared to sporadic recurrent and NBCCS-associated KCOT, which showed expression throughout all epithelial layers. There were no differences in β-catenin expression among recurrent and NBCCS-associated KCOT, whereas the intensity of survivin staining was higher in NBCCS-KCOT (P=0.0003). Nuclear staining for β-catenin was found exclusively in recurrent (5/9 cases) and NBCCS-associated (4/12 cases) KCOT.

CONCLUSION:

The data demonstrate β-catenin delocalization and survivin over-expression in recurrent sporadic and NBCCS-associated KCOT suggesting that these pathways related to apoptotic inhibition have a role in KCOT growth and recurrence.

• Authors: R Leonardi, J B Matthews, C Loreto, G Musumeci, G Campisi, L Lo Muzio, J N dos Santos, L Pastorino, P Bufo, G Pannone
• Journal: Histology and histopathology (Histol Histopathol) Vol. 28 Issue 9 Pg. 1175-84 (09 2013) ISSN: 1699-5848 [Electronic] Spain
• PMID: 23572266 (Publication Type: Comparative Study, Journal Article)

Chemical References

• BIRC5 protein, human
• CTNNB1 protein, human
• Inhibitor of Apoptosis Proteins
• Survivin
• beta Catenin
• Keratins

Topics

• Adolescent
• Adult
• Apoptosis
• Basal Cell Nevus Syndrome (metabolism)
• Cell Cycle
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Inhibitor of Apoptosis Proteins (metabolism)
• Keratins (chemistry)
• Male
• Middle Aged
• Odontogenic Tumors (metabolism)
• Recurrence
• Survivin
• Young Adult
• beta Catenin (metabolism)