Long-term ethanol exposure-induced hepatocellular carcinoma cell migration and invasion through lysyl oxidase activation are attenuated by combined treatment with pterostilbene and curcumin analogues.

Abstract	Ethanol consumption induces hepatocellular carcinoma (HCC) cell metastasis by changing the extracellular matrix (ECM). Lysyl oxidase (LOX) catalyzes the cross-linkage of collagen or elastin in the ECM. LOX protein and mRNA overexpression (>21-fold compared with controls, n = 6) was detected in cirrhotic HCC patients with a history of alcoholism. LOX protein expression was induced in HCC cells after long-term treatment with ethanol (10 mM) for 20-40 passages (denoted E20-E40 cells). Pterostilbene (PSB, 1 μM) displayed significant potency to reduce LOX-mediated activity in E40 cells when combined with curcumin and its analogues. The ability of E40 cells to form colonies in soft agar was reduced by both genetic depletion of LOX and by chemical inhibitors of LOX expression. This study suggests that targeting LOX expression with food components such as PSB and curcumin may be a novel strategy to overcome ethanol-induced HCC cell metastasis in liver cancer patients.
Authors	Ching-Shui Huang, Chi-Tang Ho, Shih-Hsin Tu, Min-Hsiung Pan, Chien-Hui Chuang, Hui-Wen Chang, Chien-Hsi Chang, Chih-Hsiung Wu, Yuan-Soon Ho
Journal	Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 61 Issue 18 Pg. 4326-35 (May 08 2013) ISSN: 1520-5118 [Electronic] United States
PMID	23560895 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents RNA, Messenger Stilbenes pterostilbene Ethanol Collagen Elastin Protein-Lysine 6-Oxidase Curcumin
Topics	Adult Aged Alcoholism Antineoplastic Agents (pharmacology) Carcinoma, Hepatocellular (pathology) Cell Line, Tumor Cell Movement (drug effects) Collagen (genetics, metabolism) Curcumin (pharmacology) Elastin (genetics, metabolism) Ethanol (adverse effects) Extracellular Matrix (drug effects, metabolism) Female Gene Expression Regulation Humans Laser Capture Microdissection Liver Neoplasms (pathology) Male Middle Aged Protein-Lysine 6-Oxidase (genetics, metabolism) RNA, Messenger (genetics, metabolism) Real-Time Polymerase Chain Reaction Stilbenes (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!