Abstract | BACKGROUND: METHODS: RESULTS: Northern blot densitometry showed liver mRNA levels an average of 12-fold greater than levels in the brain or kidney in both Lines A and D (different copies of the transgene) with no quantifiable differences between wild-type (WT) and homozygous (HO) transgenic mice. Immunoblots showed liver AGT protein levels 3.2-fold greater than levels in the brain or kidney, with no differences observed between WT and HO transgenic mice. CONCLUSION: ECFP/AngII transgene expression does not alter AGT mRNA or protein levels in major organs (kidney, liver, and brain) of transgenic mice. The altered blood pressure and kidney thrombosis observed in these transgenic mouse lines are not the result of increased intracellular AGT synthesis and resultant increases in free extracellular AngII. This finding is consistent with our published studies that indicate no increase in circulating AngII by radioimmunoassay.
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Authors | Akannsha Singh, Jason Vitko, Richard N Re, Julia L Cook |
Journal | The Ochsner journal
(Ochsner J)
Vol. 13
Issue 1
Pg. 37-41
( 2013)
ISSN: 1524-5012 [Print] United States |
PMID | 23532622
(Publication Type: Journal Article)
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