Abstract |
The aim of this study was to investigate the role of methylenetetrahydrofolate reductase (MTHFR) polymorphisms and MTHFR methylation pattern in cervical lesions development among women from Romania, a country with high prevalence of human papillomavirus (HPV) cervical infections. To achieve this goal, blood samples and cervical cytology specimens (n = 77)/tumour tissue specimens (n = 23) were investigated. As control, blood and negative cytological smears (n = 50) were used. A statistically significant association was found between T allele of C677T polymorphism and cervical lesions, heterozygote women presenting a threefold increased risk (normal/cervical lesions and tumours: wild homozygote 34/41 (0.68/0.41), heterozygote 14/51 (0.28/0.51), mutant homozygote 2/8 (0.04/0.08); OR = 3.081, P = 0.0035). Using χ square test for the control group, the HPV-negative and HPV-positive patients with cervix lesions, a significant correlation between viral infection and T allele of C677T polymorphism (P = 0.0287) was found. The MTHFR promoter was methylated in all HGSIL and tumour samples, significant differences being noted between HPV-positive samples, control group and cases of cervical dysplastic lesions without HPV DNA (P < 0. 0001) and between samples from patients with high-risk (hr)HPV versus low-risk (lr)HPV (P = 0.0026). No correlations between polymorphisms and methylation were observed. In Romania, individuals carrying T allele are susceptible for cervical lesions. MTHFR promoter methylation is associated with cervical severity lesions and with hrHPV.
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Authors | Anca Botezatu, Demetra Socolov, Iulia V Iancu, Irina Huica, Adriana Plesa, Carmen Ungureanu, Gabriela Anton |
Journal | Journal of cellular and molecular medicine
(J Cell Mol Med)
Vol. 17
Issue 4
Pg. 543-9
(Apr 2013)
ISSN: 1582-4934 [Electronic] England |
PMID | 23444906
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 The Authors. Published by Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd. |
Chemical References |
- MTHFR protein, human
- Methylenetetrahydrofolate Reductase (NADPH2)
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Topics |
- Adolescent
- Adult
- Alphapapillomavirus
(genetics)
- Carcinoma, Squamous Cell
(enzymology, genetics, virology)
- DNA Methylation
- Female
- Gene Frequency
- Genetic Association Studies
- Humans
- Methylenetetrahydrofolate Reductase (NADPH2)
(genetics)
- Middle Aged
- Papillomavirus Infections
(enzymology, genetics, virology)
- Polymorphism, Single Nucleotide
- Promoter Regions, Genetic
- Sequence Analysis, DNA
- Uterine Cervical Neoplasms
(enzymology, genetics, virology)
- Young Adult
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