Abstract |
How persistent viral infections are established and maintained is widely debated and remains poorly understood. We found here that the persistence of RNA viruses in Drosophila melanogaster was achieved through the combined action of cellular reverse-transcriptase activity and the RNA-mediated interference (RNAi) pathway. Fragments of diverse RNA viruses were reverse-transcribed early during infection, which resulted in DNA forms embedded in retrotransposon sequences. Those virus- retrotransposon DNA chimeras produced transcripts processed by the RNAi machinery, which in turn inhibited viral replication. Conversely, inhibition of reverse transcription hindered the appearance of chimeric DNA and prevented persistence. Our results identify a cooperative function for retrotransposons and antiviral RNAi in the control of lethal acute infection for the establishment of viral persistence.
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Authors | Bertsy Goic, Nicolas Vodovar, Juan A Mondotte, Clément Monot, Lionel Frangeul, Hervé Blanc, Valérie Gausson, Jorge Vera-Otarola, Gael Cristofari, Maria-Carla Saleh |
Journal | Nature immunology
(Nat Immunol)
Vol. 14
Issue 4
Pg. 396-403
(Apr 2013)
ISSN: 1529-2916 [Electronic] United States |
PMID | 23435119
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Small Interfering
- Retroelements
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Topics |
- Animals
- Base Sequence
- Cell Line
- DNA Viruses
(chemistry, genetics, metabolism)
- Disease Models, Animal
- Drosophila melanogaster
(genetics, virology)
- Female
- Gene Order
- Models, Biological
- Molecular Sequence Data
- RNA Interference
- RNA Virus Infections
(virology)
- RNA Viruses
(chemistry, genetics, metabolism)
- RNA, Small Interfering
(genetics)
- Retroelements
- Reverse Transcription
- Viral Load
- Virus Replication
(genetics)
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