Abstract | OBJECTIVES: METHODS: Forty patients with NSCLC underwent preoperative positron emission tomography-computed tomography and mediastinoscopy. Real-time polymerase chain reaction (RT-PCR) assays for messenger RNA expression of epithelial markers (ie, cytokeratin 7; carcinoembryonic antigen-related cell adhesion molecule 5; and palate, lung, and nasal epithelium carcinoma-associated protein) were performed in selected fluorodeoxyglucose-avid lymph nodes. VEGF-A, VEGF-C, VEGF-D, and VEGF receptor-3 expression levels were measured in primary tumors and lymph nodes. Wilcoxon rank sum test was run for the association between the RT-PCR epithelial marker levels and VEGF expression levels in the lymph nodes. RESULTS: CONCLUSIONS: RT-PCR analysis of fluorodeoxyglucose-avid lymph nodes results in up-staging a patient's cancer. Micrometastases correlate with the expression of VEGF in lymph nodes in patients with NSCLC. This may reflect the role of lymphangiogenesis in promoting metastases.
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Authors | Chukwumere E Nwogu, Sai Yendamuri, Wei Tan, Eric Kannisto, Paul Bogner, Carl Morrison, Richard Cheney, Elisabeth Dexter, Anthony Picone, Mark Hennon, Alan Hutson, Mary Reid, Alex Adjei, Todd L Demmy |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 145
Issue 3
Pg. 702-7; discussion 707-8
(Mar 2013)
ISSN: 1097-685X [Electronic] United States |
PMID | 23414988
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 The American Association for Thoracic Surgery. Published by Mosby, Inc. All rights reserved. |
Chemical References |
- Antigens, Tumor-Associated, Carbohydrate
- Biomarkers, Tumor
- Carcinoembryonic Antigen
- Keratin-7
- Radiopharmaceuticals
- Vascular Endothelial Growth Factor A
- Fluorodeoxyglucose F18
- Receptors, Vascular Endothelial Growth Factor
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Topics |
- Aged
- Aged, 80 and over
- Antigens, Tumor-Associated, Carbohydrate
(metabolism)
- Biomarkers, Tumor
(metabolism)
- Carcinoembryonic Antigen
(metabolism)
- Female
- Fluorodeoxyglucose F18
- Humans
- Keratin-7
(metabolism)
- Lung Neoplasms
(metabolism, pathology)
- Lymph Nodes
(metabolism, pathology)
- Lymphangiogenesis
- Lymphatic Metastasis
(pathology)
- Male
- Middle Aged
- Multimodal Imaging
- Neoplasm Micrometastasis
(pathology)
- Neoplasm Staging
- Positron-Emission Tomography
- Radiopharmaceuticals
- Real-Time Polymerase Chain Reaction
- Receptors, Vascular Endothelial Growth Factor
(metabolism)
- Statistics, Nonparametric
- Tomography, X-Ray Computed
- Vascular Endothelial Growth Factor A
(metabolism)
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