Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus.

Abstract	Prominin-1 (CD133) is commonly used to isolate stem and progenitor cells from the developing and adult nervous system and to identify cancer stem cells in brain tumors. However, despite extensive characterization of Prominin-1(+) precursor cells from the adult subventricular zone, no information about the expression of Prominin-1 by precursor cells in the subgranular zone (SGZ) of the adult hippocampus has been available. We show here that Prominin-1 is expressed by a significant number of cells in the SGZ of adult mice in vivo and ex vivo, including postmitotic astrocytes. A small subset of Prominin-1(+) cells coexpressed the nonspecific precursor cell marker Nestin as well as GFAP and Sox2. Upon fluorescence-activated cell sorting, only Prominin-1/Nestin double-positive cells fulfilled the defining stem cell criteria of proliferation, self-renewal, and multipotentiality as assessed by a neurosphere assay. In addition, isolated primary Prominin-1(+) cells preferentially migrated to the neurogenic niche in the SGZ upon transplantation in vivo. Finally, despite its expression by various stem and progenitor cells, Prominin-1 turned out to be dispensable for precursor cell proliferation in vitro and in vivo. Nevertheless, a net decrease in hippocampal neurogenesis, by ∼30% was found in Prominin-1 knock-out mice, suggesting other roles in controlling adult hippocampal neurogenesis. Remarkably, an upregulation of Prominin-2 was detected in Prominin-1-deficient mice highlighting a potential compensatory mechanism, which might explain the lack of severe symptoms in individuals carrying mutations in the Prom1 gene.
Authors	Tara L Walker, Ann Wierick, Alex M Sykes, Ben Waldau, Denis Corbeil, Peter Carmeliet, Gerd Kempermann
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci) Vol. 33 Issue 7 Pg. 3010-24 (Feb 13 2013) ISSN: 1529-2401 [Electronic] United States
PMID	23407958 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	AC133 Antigen Antigens, CD Antimetabolites DNA, Complementary Glycoproteins Intermediate Filament Proteins Membrane Glycoproteins Nerve Tissue Proteins Nes protein, mouse Nestin Peptides Prom1 protein, mouse Prom2 protein, mouse Protein Isoforms RNA Bromodeoxyuridine
Topics	AC133 Antigen Adult Stem Cells (drug effects) Animals Antigens, CD (genetics, metabolism) Antimetabolites Bromodeoxyuridine Cell Adhesion Cell Cycle (drug effects) Cell Differentiation Cell Proliferation Cell Separation (methods) DNA, Complementary (biosynthesis) Dentate Gyrus (metabolism) Flow Cytometry Glycoproteins (genetics, metabolism) Hippocampus (cytology, drug effects) Immunohistochemistry Intermediate Filament Proteins (genetics, metabolism) Membrane Glycoproteins (biosynthesis, genetics) Mice Mice, Inbred C57BL Mice, Knockout Nerve Tissue Proteins (genetics, metabolism) Nestin Neural Stem Cells (drug effects) Peptides (genetics, metabolism) Protein Isoforms RNA (isolation & purification) Real-Time Polymerase Chain Reaction

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