Human interleukin 2 receptor beta-chain gene: chromosomal localization and identification of 5' regulatory sequences.

Abstract

Interleukin 2 (IL-2) binds to and stimulates activated T cells through high-affinity IL-2 receptors (IL-2Rs). Such receptors represent a complex consisting of at least two proteins, the 55-kDa IL-2R alpha chain and the 70-kDa IL-2R beta chain. The low-affinity, IL-2R alpha chain cannot by itself transduce a mitogenic signal, whereas IL-2 stimulates resting lymphocytes through the intermediate-affinity, IL-2R beta receptor. We report here identification of the genomic locus for IL-2R beta. The exons are contained on four EcoRI fragments of 1.1, 9.2, 7.2, and 13.7 kilobases. The 1.1-kilobase EcoRI fragment lies at the 5'-most end of the genomic locus and contains promoter sequences. The promoter contains no TATA box-like elements but does contain the d(GT)n class of middle repetitive elements, which may play an interesting regulatory role. The IL-2R beta gene is localized to chromosome 22q11.2-q12, a region that is the locus for several lymphoid neoplasias.

• Authors: J R Gnarra, H Otani, M G Wang, O W McBride, M Sharon, W J Leonard
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 87 Issue 9 Pg. 3440-4 (May 1990) ISSN: 0027-8424 [Print] United States
• PMID: 2333293 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Macromolecular Substances
• Oligonucleotide Probes
• Receptors, Interleukin-2

Topics

• Base Sequence
• Blotting, Southern
• Chromosome Banding
• Chromosome Mapping
• Chromosomes, Human, Pair 22
• Exons
• Gene Library
• Genes
• Genomic Library
• Humans
• Karyotyping
• Macromolecular Substances
• Molecular Sequence Data
• Oligonucleotide Probes
• Promoter Regions, Genetic
• Receptors, Interleukin-2 (genetics)
• Regulatory Sequences, Nucleic Acid
• Restriction Mapping