Abstract | INTRODUCTION: METHODS: HK2 cells (ATCC 2290) were cultured in DMEM/HAM F12 medium, pH 7.4. At 80% confluence, after 24 hr under serum free conditions, cells were distributed in three groups (24 hours): A) Control: pH 7.4, B) Acidosis: pH 7.0 and C) Calcium citrate (0.2 mmol/L) + pH 7.0. Change (Δ) of intracellular calcium concentration, basal and after Angiotensin II (10-6M) exposition, were measured to evaluate cellular performance. EMT was evaluated by the expression of α-smooth muscle actin (α-SMA) and E-cadherin by immunocytochemistry and/or Western blot. TGF-β1 secretion was determined by ELISA in cell supernatant. RESULTS: At pH 7.0 HK2 cells significantly reduced E-cadherin and increased α-SMA expression (EMT). Supernatant TGF-β1 levels were higher than in control group. Calcium citrate decreased acidosis induced EMT and improved cells performance, without reduction of TGF-β production. CONCLUSIONS:
Acidosis induces EMT and secretion of TGF-β1 in tubular proximal cells in culture and citrate improves cellular performance and ameliorates acidosis induced EMT.
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Authors | Maria José Rodriguez Cabalgante, Liliana Gadola, Leonella Luzardo, María Márquez, José Boggia, Mirian Aparecida Boim |
Journal | Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
(J Bras Nefrol)
2012 Oct-Dec
Vol. 34
Issue 4
Pg. 343-8
ISSN: 2175-8239 [Electronic] Brazil |
PMID | 23318822
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Acidosis, Renal Tubular
(drug therapy, pathology)
- Calcium Citrate
(pharmacology, therapeutic use)
- Cells, Cultured
- Epithelial-Mesenchymal Transition
(drug effects)
- Humans
- Kidney Tubules, Proximal
(cytology, drug effects)
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