Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-γ) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population.

Abstract

BACKGROUND:

chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-γ and its receptor (IFN-γ R) are critical in the pathogenesis of infection.

OBJECTIVES:

The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-γ R1) and chronic HBV infection.

MATERIALS AND METHODS:

Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.

RESULTS:

The results indicated that both SNP's frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.

CONCLUSIONS:

Presence of G allele at position -611 within promoter of IFN-γ R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.

• Authors: Sayyad Khanizadeh, Mehrdad Ravanshad, Seyed Reza Mohebbi, Hamed Naghoosi, Mohamad Abrahim Tahaei, Seyed Dawood Mousavi Nasab, Sara Romani, Pedram Azimzadeh, Azar Sanati, Mohammad Reza Zali
• Journal: Hepatitis monthly (Hepat Mon) Vol. 12 Issue 11 Pg. e7283 (Nov 2012) ISSN: 1735-143X [Print] Netherlands
• PMID: 23300496 (Publication Type: Journal Article)