The protective effect of hyperbaric oxygen and Ginkgo biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats.

Abstract

Alzheimer's disease (AD) is characterized by accumulation and deposition of Aβ peptides in human brains. The present study aimed to determine the protective effect of HBO and EGB761 on Aβ25-35 peptides induced cognitive impairment and neuronal toxicity in rats. Characteristics of AD were induced in rats by the administration of Aβ25-35 in hippocampus. Rats were treated with HBO (2ATA 60min/day), EGB761 (20mg/kg/day), and the combination of HBO+EGB761 (20mg/kg/day+2ATA). The Morris water maze was used to detect the protective effects of HBO and EGB761 against cognitive impairment. The activities of SOD and GSH, the apoptosis-related genes and proteins and the apoptosis rate of hippocampus were detected. Compared to the model group, EGB761 and HBO treatments synergistically improved the escape latency. Furthermore, the activities of SOD and GSH in rat hippocampal tissue were found to have increased with a concomitant reduction in MDA levels, Bax expression, cytochrome c release, and the activity of caspase-9/3. Accordingly, a significant reduction was observed in the apoptosis rate following the treatment with EGB761 and HBO in this model of AD. Our findings suggest that HBO and EGB761 reduce cell toxicity and oxidative stress by blocking mitochondria-mediated apoptosis signaling in AD, and the combined treatment of HBO and Ginkgo further enhances these effects.

• Authors: Xiaoqiang Tian, Lida Zhang, Jingmei Wang, Jianguo Dai, Shanshan Shen, Lin Yang, Peilin Huang
• Journal: Behavioural brain research (Behav Brain Res) Vol. 242 Pg. 1-8 (Apr 01 2013) ISSN: 1872-7549 [Electronic] Netherlands
• PMID: 23266522 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Neuroprotective Agents
• Peptide Fragments
• Plant Extracts
• RNA, Messenger
• Reactive Oxygen Species
• amyloid beta-protein (25-35)
• bcl-2-Associated X Protein
• Cyclin D1
• Ginkgo biloba extract
• Caspases

Topics

• Alzheimer Disease (chemically induced, pathology, therapy)
• Amyloid beta-Peptides (pharmacology)
• Analysis of Variance
• Animals
• Apoptosis (drug effects)
• Caspases (metabolism)
• Cyclin D1 (genetics, metabolism)
• Disease Models, Animal
• Gene Expression Regulation (drug effects)
• Ginkgo biloba
• Hippocampus (cytology)
• Hyperbaric Oxygenation
• In Situ Nick-End Labeling
• Male
• Maze Learning (drug effects)
• Motor Activity (drug effects)
• Neurons (drug effects)
• Neuroprotective Agents (pharmacology)
• Oxidative Stress (drug effects)
• Peptide Fragments (pharmacology)
• Plant Extracts (pharmacology)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reaction Time (drug effects)
• Reactive Oxygen Species (metabolism)
• Time Factors
• bcl-2-Associated X Protein (genetics, metabolism)