Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model.

Abstract

We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization.

• Authors: Violeta Dancheva, Lyudmil Terziev, Veneta Shopova, Galya Stavreva
• Journal: Iranian journal of allergy, asthma, and immunology (Iran J Allergy Asthma Immunol) Vol. 11 Issue 4 Pg. 329-35 (Dec 2012) ISSN: 1735-1502 [Print] Iran
• PMID: 23264410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Metalloporphyrins
• Mn(III) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin
• Sulfhydryl Compounds
• Ovalbumin
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Animals
• Antioxidants (administration & dosage, metabolism, pharmacology)
• Asthma (drug therapy, immunology, metabolism)
• Catalase (metabolism)
• Disease Models, Animal
• Female
• Glutathione Peroxidase (metabolism)
• Injections, Intraperitoneal
• Lung (drug effects, immunology, metabolism)
• Metalloporphyrins (administration & dosage, pharmacology)
• Mice
• Mice, Inbred C57BL
• Ovalbumin
• Oxidative Stress (drug effects)
• Sulfhydryl Compounds (metabolism)
• Superoxide Dismutase (metabolism)
• Time Factors