Discovery and verification of panels of T-lymphocyte proteins as biomarkers of Parkinson's disease.

Abstract

The diagnosis of Parkinson's disease (PD) is currently based on the clinical evaluation of extrapyramidal signs with a considerable error rate. The identification of specific markers might allow PD diagnosis before the onset of classical motor symptoms. By two-dimensional electrophoresis we identified proteome alterations in T-lymphocytes of 17 control subjects and 15 PD patients. The observed changes were used to build predictive models that were verified by the leave-one-out cross-validation. We further built two functions able to stage the subjects. We chose to verify by Western blotting the identity of spots corresponding to β-fibrinogen and transaldolase, two recurrent proteins in six out of 20 spots. β-Fibrinogen levels are lowered in PD patients, whereas a heavy transaldolase set of isoforms was more abundant. Eventually, we identified a list of seven proteins showing different levels in early-onset with respect to late-onset PD patients.

• Authors: Tiziana Alberio, Agnese C Pippione, Maurizio Zibetti, Simone Olgiati, Daniela Cecconi, Cristoforo Comi, Leonardo Lopiano, Mauro Fasano
• Journal: Scientific reports (Sci Rep) Vol. 2 Pg. 953 ( 2012) ISSN: 2045-2322 [Electronic] England
• PMID: 23233872 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Protein Isoforms
• Proteome
• Fibrinogen
• Transaldolase

Topics

• Biomarkers (analysis)
• Blotting, Western
• Electrophoresis, Gel, Two-Dimensional
• Fibrinogen (analysis)
• Humans
• Parkinson Disease (diagnosis)
• Protein Isoforms (analysis)
• Proteome (analysis)
• T-Lymphocytes (chemistry)
• Transaldolase (analysis)