Abstract | BACKGROUND AND OBJECTIVE:
Photodynamic therapy ( PDT) has become one of the emerging options in management of cancer and other diseases. The major goal of PDT is to kill cancer cell without causing any adverse effect to the normal cells. PDT in combination of different therapeutic agents is being evaluated to improve the efficacy of treatment. Genistein, a soy ingredient, has widely been studied against different types of cancer. In the present study, combination of these two therapeutic methods has been studied to evaluate the enhanced effectiveness and find out the mechanism of action. MATERIALS AND METHODS: RESULTS: From the result, the combination of genistein and photofrin mediated PDT enhanced the apoptotic effect against SNU 80 cells. Proliferation of the cells was inhibited and the mitochondrial membrane depolarisation was observed. ROS level were also increased in combination treatment. The expressions of Caspase 3, Caspase 9, cytochrome c, Caspase 8, Caspae 12 and other apoptosis related proteins were also modified. CONCLUSION: Thus PDT can induce apoptosis in thyroid cancer cells singly or in combination with genistein. But in combination treatment, the efficacy of inducing apoptosis in SNU 80 cells is much higher than that of individual treatment with genistein or PDT.
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Authors | Jin-Chul Ahn, Raktim Biswas, Phil-Sang Chung |
Journal | Lasers in surgery and medicine
(Lasers Surg Med)
Vol. 44
Issue 10
Pg. 840-9
(Dec 2012)
ISSN: 1096-9101 [Electronic] United States |
PMID | 23143780
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Wiley Periodicals, Inc. |
Chemical References |
- Antineoplastic Agents
- Photosensitizing Agents
- Dihematoporphyrin Ether
- Genistein
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Topics |
- Antineoplastic Agents
(therapeutic use)
- Antineoplastic Combined Chemotherapy Protocols
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Blotting, Western
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Dihematoporphyrin Ether
(pharmacology, therapeutic use)
- Genistein
(pharmacology, therapeutic use)
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Microscopy, Confocal
- Photochemotherapy
- Photosensitizing Agents
(pharmacology, therapeutic use)
- Thyroid Carcinoma, Anaplastic
- Thyroid Neoplasms
(drug therapy)
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