Inhibitory effects of wasabi isothiocyanates on chemical mediator release in RBL-2H3 rat basophilic leukemia cells.

Abstract

Wasabi is a plant of Japanese origin. It belongs to the family Brassicaceae and produces various isothiocyanates (ITCs). To clarify the type I allergies inhibited by wasabi ITCs, we investigated the inhibitory effect on chemical mediator release from dinitrophenylated bovine serum albumin (DNP-BSA)-stimulated RBL-2H3 rat basophilic leukemia cells. Allyl ITC (AITC), sec-butyl ITC (s-BuITC), and 3-butenyl ITC (3-BuITC), which have 3 or 4 carbon chains, inhibited histamine release but did not inhibit the release of leukotriene B4 (LTB4) or cysteinyl LTs (CysLTs). 4-Pentenyl ITC (4-PeITC) and 5-hexenyl ITC (5-HeITC), which have 5 or 6 carbon chains and an unsaturated bond at the end, inhibited LTB4 release but did not inhibit the release of histamine or CysLTs. 6-Methylthiohexyl ITC (6-MTITC), 6-methylsulfinylhexyl ITC (6-MSITC), and 6-methylsulfonylhexyl ITC (6-MSFITC), which have a sulfur atom inserted at the end of a 6-carbon chain, inhibited the release of histamine, LTB4, and CysLTs and the elevation in intracellular Ca(2+). These results suggest that wasabi ITCs inhibited type I allergies by inhibiting chemical mediator release and that the inhibitory effects on each chemical mediator were due to differences in the side chain structure of the wasabi ITCs.

• Authors: Tomoe Yamada-Kato, Masashi Nagai, Motoko Ohnishi, Kazutoshi Yoshida
• Journal: Journal of nutritional science and vitaminology (J Nutr Sci Vitaminol (Tokyo)) Vol. 58 Issue 4 Pg. 303-7 ( 2012) ISSN: 1881-7742 [Electronic] Japan
• PMID: 23132316 (Publication Type: Journal Article)

Chemical References

• 6-methylsulfinylhexyl isothiocyanate
• Dinitrophenols
• Isothiocyanates
• Plant Extracts
• dinitrophenyl-bovine serum albumin
• Leukotriene B4
• Serum Albumin, Bovine
• 2,3,4-tri-O-acetylarabinopyranosyl isothiocyanate
• 6-methylthiohexyl isothiocyanate

Topics

• Animals
• Cattle
• Cell Line, Tumor
• Dinitrophenols (metabolism)
• Histamine Release (drug effects)
• Isothiocyanates (pharmacology)
• Leukemia, Basophilic, Acute (pathology)
• Leukotriene B4 (antagonists & inhibitors, metabolism)
• Plant Extracts (pharmacology)
• Rats
• Serum Albumin, Bovine (metabolism)
• Wasabia (chemistry)