Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.

Abstract

BACKGROUND:

To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed.

MATERIAL/METHODS:

Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21 kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (β2M).

RESULTS:

The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and β2M were decreased.

CONCLUSIONS:

The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.

• Authors: Jae Ho Kim, Sang Kwang Lee, Yong Cheol Yoo, Nam Hyun Park, Dan Bi Park, Jong Shin Yoo, Hyun Joo An, Young Mok Park, Kyung Gi Cho
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 18 Issue 11 Pg. BR450-60 (Nov 2012) ISSN: 1643-3750 [Electronic] United States
• PMID: 23111736 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Neoplasm Proteins
• Proteome

Topics

• Adult
• Aged
• Amino Acid Sequence
• Biomarkers, Tumor (cerebrospinal fluid)
• Blotting, Western
• Electrophoresis, Gel, Two-Dimensional
• Female
• Humans
• Immunohistochemistry
• Male
• Meningeal Neoplasms (cerebrospinal fluid, diagnosis)
• Meningioma (cerebrospinal fluid, diagnosis)
• Middle Aged
• Molecular Sequence Annotation
• Molecular Sequence Data
• Neoplasm Proteins (cerebrospinal fluid, chemistry)
• Proteome (chemistry, metabolism)
• Proteomics (methods)
• Reproducibility of Results
• Spectrometry, Mass, Electrospray Ionization