Abstract | BACKGROUND: Flow cytometry (FCM) evaluating DNA content is emerging as the tool to monitor cell proliferation and malignant potential in several cancers such as stomach, lung, and salivary gland tumor. The purpose of this study was to correlate (18)F-FDG uptake of dual-time-point (DTP) positron emission tomography (PET) imaging with DNA ploidy and S-phase fraction (SPF) in primary breast cancer lesions. PATIENTS AND METHODS: Seventy-two consecutive female patients (mean age ± SD, 52.7 ± 11.1 years; range, 28-81 years) had undergone (18)F-FDG DTP PET/computed tomography (CT) imaging for staging of breast malignancy in our institution during a 5-month period. FCM was performed on fresh-frozen samples of specimens obtained from surgery. (18)F-FDG uptake was then compared with DNA content. RESULTS: Forty-four malignant lesions were included in this study. On FCM, DNA aneuploidy was detected in 14 lesions (31.8%). The maximum standardized uptake values (SUV(max1) and SUV(max2)) (SUV(max1), 6.8 ± 4.6 vs. 3.4 ± 2.4; P = .017), (SUV(max2), 7.9 ± 5.7 vs. 3.6 ± 2.7; P = .015) and retention index (RI) (12.8 ± 11.6 vs. 2.4 ± 10.8; P = .010) were significantly higher in DNA aneuploidy cancer than in DNA diploidy cancer. The value of RI (11.3 ± 11.5 vs. 2.6 ± 11.2; P = .022) was significantly higher in high SPF (> 15%) breast cancer than in low SPF (≤ 15%) breast cancer. CONCLUSIONS:
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Authors | Dae-Weung Kim, Chang Guhn Kim |
Journal | Clinical breast cancer
(Clin Breast Cancer)
Vol. 13
Issue 3
Pg. 196-201
(Jun 2013)
ISSN: 1938-0666 [Electronic] United States |
PMID | 23083500
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Radiopharmaceuticals
- Fluorodeoxyglucose F18
- DNA
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Breast Neoplasms
(diagnostic imaging, genetics, metabolism)
- DNA
- Female
- Fluorodeoxyglucose F18
(pharmacokinetics)
- Humans
- Middle Aged
- Ploidies
- Positron-Emission Tomography
- Radiopharmaceuticals
(pharmacokinetics)
- S Phase
- Tomography, X-Ray Computed
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