Abstract |
Pulmonary intravascular macrophages (PIMs) are present in species such as cattle, sheep and horse and promote acute lung inflammation (ALI). Rabbits are often used as a model of ALI but there is controversy about the presence of PIMs in these species. Rabbits were treated with 10 mg/kg of gadolinium chloride intravenously (GC; n = 6) or saline (n = 6) followed by euthanasia at 48 h post-treatment to determine the presence of PIMs. In a subsequent study, rabbits were pre-treated with GC or 0.9 % saline followed by 100 μg/kg of E. coli lipopolysaccharide intravenously 48 h later. Rabbits were euthanized 24 h post-LPS treatment. Light and electron microscopy showed that PIMs attached to the capillary endothelium and were positive for RAM-11 anti-macrophage antibody. While GC treatment induced apoptotic PIMs, there was no difference in the PIM number between control and GC-treated rabbits. Rabbits administered with LPS were 3.5 times more likely to die before the end of the 24-h period than those pre-treated with GC. Lung heterophil accumulation and IL-1β, TNFα and IL-6 mRNA expression were significantly higher in rabbits administered with LPS compared to those administered with GC before the LPS injection. PIMs from the LPS-treated rabbits were positive for TNFα. Lung, BAL and serum IL-8 and MCP-1 expression was not different between LPS rabbits with or without pre-treatment with GC. We conclude that rabbit lungs contain PIMs and that their depletion reduces endotoxin-induced lung inflammation. The presence of PIMs in rabbit lungs may need to be considered while using rabbit to model acute lung injury.
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Authors | Tanya Duke-Novakovski, Sarabjeet Singh-Suri, Osamu Kajikawa, Sarah Caldwell, Chandarshekhar Charavaryamath, Baljit Singh |
Journal | Cell and tissue research
(Cell Tissue Res)
Vol. 351
Issue 1
Pg. 149-60
(Jan 2013)
ISSN: 1432-0878 [Electronic] Germany |
PMID | 23073615
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endotoxins
- Interleukin-1beta
- Interleukin-6
- Lipopolysaccharides
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- Gadolinium
- Peroxidase
- gadolinium chloride
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Topics |
- Animals
- Endotoxins
(toxicity)
- Gadolinium
(pharmacology)
- Immunophenotyping
- Interleukin-1beta
(genetics, metabolism)
- Interleukin-6
(genetics, metabolism)
- Lipopolysaccharides
(pharmacology)
- Lung
(blood supply, drug effects, enzymology)
- Macrophages, Alveolar
(drug effects, immunology, ultrastructure)
- Peroxidase
(metabolism)
- RNA, Messenger
(blood, genetics)
- Rabbits
- Reverse Transcriptase Polymerase Chain Reaction
- Sputum
(cytology, drug effects)
- Survival Analysis
- Tumor Necrosis Factor-alpha
(genetics, metabolism)
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