Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis.

Abstract

The purpose of this study was to investigate the activities of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E-ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E-NTPDase and E-ADA activities were assayed. The results demonstrated an increased E-NTPDase activity (both ATP and ADP as substrates) and a decreased E-ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti-inflammatory and analgesic properties, working as a potent suppressor of immune response.

• Authors: Jeandre Augusto Dos Santos Jaques, Lara Vargas Becker, Viviane do Carmo Gonçalves Souza, Cláudio Alberto Martins Leal, Tatiana Montagner Dalcin Bertoldo, Kelly de Vargas Pinheiro, Vera Maria Morsch, Maria Rosa Chitolina Schetinger, Daniela Bitencourt Rosa Leal
• Journal: Cell biochemistry and function (Cell Biochem Funct) Vol. 31 Issue 5 Pg. 395-9 (Jul 2013) ISSN: 1099-0844 [Electronic] England
• PMID: 23070807 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 John Wiley & Sons, Ltd.

Chemical References

• Antigens, CD
• Adenosine Diphosphate
• Adenosine Triphosphate
• Adenosine Deaminase
• Apyrase
• CD39 antigen

Topics

• Adenosine Deaminase (metabolism)
• Adenosine Diphosphate (metabolism)
• Adenosine Triphosphate (metabolism)
• Adult
• Antigens, CD (metabolism)
• Apyrase (metabolism)
• Arthritis, Rheumatoid (enzymology, pathology)
• Case-Control Studies
• Cells, Cultured
• Enzyme Assays
• Female
• Humans
• Lymphocytes (enzymology, pathology)
• Male
• Middle Aged