Abstract | OBJECTIVES: MATERIALS AND METHODS: DSG3 expression was determined in multiple general cancer- and HNSCC-tissue microarrays (TMAs), in negative and positive HNSCC metastatic cervical lymph nodes, and in a variety of HNSCC and control cell lines. A nanostructured immunoarray system was developed for the ultrasensitive detection of DSG3 in lymph node tissue lysates. RESULTS: We demonstrate that DSG3 is highly expressed in all HNSCC lesions and their metastatic cervical lymph nodes, but absent in non-invaded lymph nodes. We show that DSG3 can be rapidly detected with high sensitivity using a simple microfluidic immunoarray platform, even in human tissue sections including very few HNSCC invading cells, hence distinguishing between positive and negative lymph nodes. CONCLUSION: We provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis and guide in the selection of appropriate therapeutic intervention modalities for HNSCC patients.
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Authors | Vyomesh Patel, Daniel Martin, Ruchika Malhotra, Christina A Marsh, Colleen L Doçi, Timothy D Veenstra, Cherie-Ann O Nathan, Uttam K Sinha, Bhuvanesh Singh, Alfredo A Molinolo, James F Rusling, J Silvio Gutkind |
Journal | Oral oncology
(Oral Oncol)
Vol. 49
Issue 2
Pg. 93-101
(Feb 2013)
ISSN: 1879-0593 [Electronic] England |
PMID | 23010602
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural)
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Copyright | Published by Elsevier Ltd. |
Chemical References |
- Biomarkers, Tumor
- DSG3 protein, human
- Desmoglein 3
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Topics |
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Carcinoma, Squamous Cell
(pathology)
- Desmoglein 3
(metabolism)
- Humans
- Immunohistochemistry
- Lymphatic Metastasis
(diagnosis)
- Microfluidics
- Mouth Neoplasms
(pathology)
- Nanostructures
- Sensitivity and Specificity
- Tissue Array Analysis
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