MicroRNA expression profile of the hippocampus in a rat model of temporal lobe epilepsy and miR-34a-targeted neuroprotection against hippocampal neurone cell apoptosis post-status epilepticus.
Abstract | BACKGROUND: METHODS: RESULTS: The miRNA microarray detected 9 up-regulated miRNAs (miR-146a, -211, -203, -210, -152, -31, -23a, -34a, -27a) and 15 down-regulated miRNAs (miR-138*, -301a, -136, -153, -19a, -135b, -325-5p, -380, -190, -542-3p, -33, -144, -542-5p, -543, -296*). Some of the deregulated miRNAs (miR-146a, miR-210, miR-27a, miR-135b and miR-33) were confirmed using qPCR. Furthermore, an increase in expression of the pro-apoptotic miR-34a was demonstrated in the post- status epilepticus rat hippocampus. miR-34a was significantly up-regulated at 1 day, 7 days and 2 weeks post- status epilepticus and at 2 months after temporal lobe epilepsy. Experiments with the miR-34a antagomir revealed that targeting miR-34a led to an inhibition of activated caspase-3 protein expression, which may contribute to increased neuronal survival and reduced neuronal death or apoptosis. CONCLUSIONS: Our study showed the expression profile of miRNAs in the hippocampus in a rat model of temporal lobe epilepsy and an increase in the expression of the pro-apoptotic miR-34a in post- status epilepticus rats. The results show that miR-34a is up-regulated during seizure-induced neuronal death or apoptosis, and targeting miR-34a is neuroprotective and is associated with an inhibition of an increase in activated caspase-3 protein.
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Authors | Kai Hu, Yuan-Yuan Xie, Chen Zhang, Dong-Sheng Ouyang, Hong-Yu Long, Dan-Ni Sun, Li-Li Long, Li Feng, Yi Li, Bo Xiao |
Journal | BMC neuroscience
(BMC Neurosci)
Vol. 13
Pg. 115
(Sep 22 2012)
ISSN: 1471-2202 [Electronic] England |
PMID | 22998082
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN34 microRNA, rat
- MicroRNAs
- Oligonucleotides
- RNA, Messenger
- Pilocarpine
- Lithium
- Caspase 3
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Topics |
- Analysis of Variance
- Animals
- Apoptosis
(drug effects, physiology)
- Caspase 3
(genetics, metabolism)
- Cell Death
(drug effects)
- Computational Biology
- Disease Models, Animal
- Epilepsy, Temporal Lobe
(chemically induced, pathology)
- Hippocampus
(metabolism, pathology)
- In Situ Nick-End Labeling
- Lithium
(toxicity)
- Male
- MicroRNAs
(antagonists & inhibitors, genetics, metabolism)
- Microarray Analysis
- Neurons
(metabolism)
- Oligonucleotides
(pharmacology)
- Pilocarpine
(toxicity)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Status Epilepticus
(chemically induced, pathology)
- Up-Regulation
(drug effects)
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