Abstract |
Angiogenesis is a prerequisite for endometriotic lesion formation and development. Pigment epithelium-derived factor (PEDF) is a potential inhibitor of angiogenesis. The objective of this study was to detect PEDF immunolocalization in endometriotic lesions and the correlation with vascular endothelial growth factor ( VEGF) and microvascular density (MVD) in a rat model of endometriosis. A subcutaneous endometriosis rat model was established by using auto- transplantation. Expression of PEDF, VEGF and MVD labeled by von Willebrand factor (v-WF) in endometriotic lesions and endometrial tissues was evaluated using immunohistochemical staining. We detected lower PEDF immunostaining and higher VEGF and MVD immunostaining in active lesions in a rat model of endometriosis than that in endometriosis endometrium or control endometrium (P<0.05), but no differences between endometriosis and control endometrium were found (P>0.05). In lesions, PEDF expression was negatively correlated with VEGF expression, MVD or sizes of cysts (P<0.01). On the contrary, both VEGF expression and MVD were positively correlated with lesion sizes (P<0.05). In addition, VEGF expression was positively correlated with MVD (P<0.01). Our results suggest that PEDF might be involved in the pathogenesis of endometriosis and may lead to novel treatment for this disease.
|
Authors | Guofang Fu, Xuan Che, Yanmei Sun, Xiufeng Huang, Hong Xu, Caiyun Zhou, Xinmei Zhang |
Journal | Acta histochemica
(Acta Histochem)
Vol. 115
Issue 4
Pg. 301-7
(May 2013)
ISSN: 1618-0372 [Electronic] Germany |
PMID | 22975116
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 Elsevier GmbH. All rights reserved. |
Chemical References |
- Eye Proteins
- Nerve Growth Factors
- Serpins
- Vascular Endothelial Growth Factor A
- Vimentin
- pigment epithelium-derived factor
- Keratins
|
Topics |
- Animals
- Disease Models, Animal
- Endometriosis
(physiopathology)
- Endometrium
(anatomy & histology, physiopathology)
- Eye Proteins
(genetics, metabolism)
- Female
- Gene Expression Regulation
- Humans
- Immunohistochemistry
- Keratins
(metabolism)
- Nerve Growth Factors
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Serpins
(genetics, metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
- Vimentin
(metabolism)
|