Abstract | OBJECTIVES: METHODS: Samples of knee articular cartilage were taken during surgery; cartilage samples obtained from fresh cadavers without arthritic disease were used as controls. Samples were digested with collagenase; isolated chondrocytes were cultured in monolayers. Aggrecan was detected by toluidine blue staining; collagen and aggrecan protein levels were evaluated by immuno cytochemistry and immuno fluorescence staining. RESULTS: Samples were obtained from six participants per group. Aggrecan and type II collagen levels in chondrocytes from patients were significantly lower than those from controls, but levels of type I, III and X collagen were enhanced in patients compared with controls. Production of type III and X collagen was higher in chondrocytes from patients with Kashin-Beck disease than in those from OA patients. CONCLUSIONS:
|
Authors | Z Q Gao, X Guo, C Duan, W Ma, P Xu, W Wang, J C Chen |
Journal | The Journal of international medical research
(J Int Med Res)
Vol. 40
Issue 4
Pg. 1325-34
( 2012)
ISSN: 1473-2300 [Electronic] England |
PMID | 22971484
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- ACAN protein, human
- Aggrecans
- Fibrillar Collagens
|
Topics |
- Adult
- Aged
- Aggrecans
(biosynthesis)
- Cartilage, Articular
(metabolism, pathology)
- Case-Control Studies
- Chondrocytes
(metabolism)
- Female
- Fibrillar Collagens
(genetics, metabolism)
- Humans
- Kashin-Beck Disease
(metabolism, pathology)
- Male
- Middle Aged
- Osteoarthritis, Knee
(metabolism, pathology)
|