OBJECTIVES To assess the effect of
bevacizumab injection on
connective tissue growth factor (CTGF) and
vascular endothelial growth factor (
VEGF) in the ocular fluids of patients with diabetic
traction retinal detachment, and to determine whether intraoperative and postoperative complications are decreased in eyes given adjunctive preoperative
bevacizumab injection. METHODS Twenty eyes of 19 patients were randomized to receive intravitreal
bevacizumab or
sham injection 3 to 7 days before
vitrectomy for severe proliferative
diabetic retinopathy. We collected aqueous samples before injection and at the time of
vitrectomy and extracted undiluted vitreous samples. RESULTS Five eyes had decreased vascularization of membranes from preinjection to the time of
vitrectomy (all in the
bevacizumab treatment arm). Median visual acuities were 20/400 in control eyes at baseline and postoperative month 3 (POM3) and 8/200 in the
bevacizumab-treated group at baseline and 20/100 at POM3 (
P= .30 between control and
bevacizumab-treated groups at POM3). All retinas were attached at POM3. Vitreous levels of
VEGF were significantly lower in the
bevacizumab group than in the control group (P= .03). Vitreous levels of CTGF were slightly lower in the
bevacizumab group compared with the control group, but this difference was not statistically significant (P= .38). Levels of CTGF in the aqueous were strongly correlated with CTGF levels in the vitreous of controls (Spearman correlation coefficient, 0.95 [P< .001]). CONCLUSIONS Intravitreal
bevacizumab injection reduces vitreous levels of
VEGF and produces a clinically observable alteration in diabetic fibrovascular membranes. Ocular fluid levels of CTGF are not significantly affected within the week after
VEGF inhibition.
Retinal reattachment rates and visual acuity are not significantly altered by preoperative intravitreal
bevacizumab injection at POM3. TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT01270542.