Effects of schizandrin on the expression of thymic stromal lymphopoietin in human mast cell line HMC-1.

Abstract

AIMS:

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic diseases such as asthma and atopic dermatitis. Schizandrin has various effects such as anti-asthmatic, anti-cancer and anti-inflammatory effects. However, the effect of schizandrin on the production of TSLP has not been clarified. Thus, we investigated how schizandrin inhibits the production of TSLP in the human mast cell line HMC-1 cells.

MAIN METHODS:

We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and Western blot analysis to investigate the effects of schizandrin.

KEY FINDINGS:

Schizandrin inhibited the production and mRNA expression of TSLP in HMC-1 cells. The maximal inhibition rate of TSLP production by schizandrin (10 μM) was 68.62 ± 3.47%. Schizandrin inhibited the translocation and luciferase activity of nuclear factor-κB induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, the activation of caspase-1 was increased, whereas the activation of caspase-1 was decreased by pretreatment with schizandrin.

SIGNIFICANCE:

These results suggest that schizandrin can be used to treat inflammatory and atopic diseases through the inhibition of TSLP.

• Authors: Phil-Dong Moon, Hyun-Ja Jeong, Hyung-Min Kim
• Journal: Life sciences (Life Sci) Vol. 91 Issue 11-12 Pg. 384-388 (Oct 05 2012) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 22906632 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Cyclooctanes
• Cytokines
• Lignans
• Polycyclic Compounds
• Calcimycin
• Luciferases
• Caspase 1
• schizandrin
• Tetradecanoylphorbol Acetate
• Thymic Stromal Lymphopoietin

Topics

• Blotting, Western
• Calcimycin (pharmacology)
• Caspase 1 (biosynthesis)
• Cell Line
• Cyclooctanes (pharmacology)
• Cytokines (biosynthesis)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Lignans (pharmacology)
• Luciferases
• Mast Cells (drug effects, metabolism, physiology)
• Polycyclic Compounds (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Tetradecanoylphorbol Acetate (pharmacology)
• Thymic Stromal Lymphopoietin