Abstract | OBJECTIVE: METHODS: RESULTS: After dealing with high glucose/hyperinsulin for 24 h, insulin resistance was induced successfully in the 3T3-L1 adipocytes. The concentrations of UA were defined to be 4 to 20 μmol/L. Compared with the model group, the glucose uptake was significantly increased in the rosiglitazone group and groups treated with low- and high-dose UA (P<0.01). The differentiation levels of 3T3-L1 adipocytes in the UA groups were lower than those in the control group and the rosiglitazone group. Effects of UA on the expressions of aP2 and MMP-1 were not obvious, but UA could up-regulate expression of CAP both in mRNA and protein levels (P<0.01). CONCLUSION: Low- and high-dose UA can improve the glycometabolism and differentiation of 3T3-L1 adipocytes with insulin resistance by up-regulating the expression of CAP.
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Authors | Di Li, Guan-liang Wang, Meng-ya Shan, Jia-han Liu, Lin Wang, De-zeng Zhu |
Journal | Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
(Zhong Xi Yi Jie He Xue Bao)
Vol. 10
Issue 8
Pg. 886-93
(Aug 2012)
ISSN: 1672-1977 [Print] China |
PMID | 22883405
(Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Insulin
- Triterpenes
- Matrix Metalloproteinase 13
- Mmp13 protein, mouse
- ursolic acid
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Topics |
- 3T3-L1 Cells
(drug effects)
- Adipocytes
(cytology, drug effects, metabolism)
- Animals
- Cell Differentiation
- Insulin
(metabolism)
- Insulin Resistance
- Matrix Metalloproteinase 13
(metabolism)
- Mice
- Triterpenes
(pharmacology)
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