Abstract | BACKGROUND:
MicroRNAs ( miRNAs) play an essential role in mediating gene expression in both normal and malignant cells. However, little is known about specific miRNAs during the development of hepatocellular carcinoma (HCC) from well-differentiated to poorly differentiated cells. METHODS: We performed miRNA array analysis of three different HCC cell lines: HepG2, HepJ5, and skHep-1. The expression patterns of miR-200 family members were confirmed by real-time polymerase chain reaction (PCR). We overexpressed miR-200 family members by using a lentivirus system and selected for stably transduced cells using antibiotics. The migration ability of the cells was tested using the Transwell migration assay system. RESULTS: Our miRNA array and real-time PCR results indicated a decrease in the expression of miR-200 family members in poorly differentiated skHep-1 cells compared with well-differentiated HepG2 cells. We overexpressed miR-200a and miR-200b in both HepJ5 and skHep-1 cells and found that the overexpression of the miR-200 family members did not influence proliferation, although migration was decreased in these cells. We found that overexpression of miR-200 family members led to an upregulation of E-cadherin expression in both HepJ5 and skHep-1 cells. Furthermore, we silenced E-cadherin expression by shRNA in miR200a-HepJ5 cells and found that the migratory ability of these cells was enhanced upon the decrease in E-cadherin expression. CONCLUSIONS: Members of the miR-200 family (miR-200a and miR-200b) play important roles in HCC migration by regulating E-cadherin expression.
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Authors | Chin-Sheng Hung, Hui-Hsiung Liu, Jun-Jen Liu, Chi-Tai Yeh, Tung-Cheng Chang, Chih-Hsiung Wu, Yuan-Soon Ho, Po-Li Wei, Yu-Jia Chang |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 20 Suppl 3
Pg. S360-8
(Dec 2013)
ISSN: 1534-4681 [Electronic] United States |
PMID | 22868917
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Cadherins
- MIRN200 microRNA, human
- MicroRNAs
- RNA, Messenger
- RNA, Small Interfering
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Topics |
- Biomarkers, Tumor
(genetics, metabolism)
- Blotting, Western
- Cadherins
(antagonists & inhibitors, genetics, metabolism)
- Carcinoma, Hepatocellular
(genetics, metabolism, pathology)
- Cell Movement
- Cell Survival
- Epithelial-Mesenchymal Transition
- Humans
- Liver Neoplasms
(genetics, metabolism, pathology)
- MicroRNAs
(genetics)
- RNA, Messenger
(genetics)
- RNA, Small Interfering
(genetics)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Cells, Cultured
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