Evaluation of novel adjuvant Eimeria profilin complex on intestinal host immune responses against live E. acervulina challenge infection.

Abstract

The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN [T]) (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 x 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1+/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks.

• Authors: Sung Hyen Lee, Hyun S Lillehoj, Seung I Jang, Kyung Woo Lee, Duk Kyung Kim, Erik P Lillehoj, Robert J Yancey, Paul J Dominowski
• Journal: Avian diseases (Avian Dis) Vol. 56 Issue 2 Pg. 402-5 (Jun 2012) ISSN: 0005-2086 [Print] United States
• PMID: 22856201 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Protozoan
• CPG-oligonucleotide
• Glycolipids
• Oligodeoxyribonucleotides
• Profilins
• Protozoan Vaccines
• Recombinant Proteins
• Bay-R 1005

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antibodies, Protozoan (blood)
• Body Weight
• Chickens
• Coccidiosis (immunology, prevention & control, veterinary)
• Duodenum (immunology, pathology)
• Eimeria (immunology)
• Glycolipids (administration & dosage)
• Lymphocytes (immunology)
• Oligodeoxyribonucleotides (administration & dosage)
• Poultry Diseases (immunology, prevention & control)
• Profilins (immunology)
• Protozoan Vaccines (immunology)
• Recombinant Proteins (immunology)