Abstract |
To clarify involvement of complement activation in thrombus formation on polymer surfaces, in vitro complement activation was evaluated for polyethylene (PE) tubes radiation-graft copolymerized with acrylamide (AAm), acrylic acid (AC), 2-hydroxyethyl methacrylate ( HEMA), N-vinylpyrrolidone (NVP), and vinyl alcohol (VOH), and compared to their in vivo antithrombogenicity and cell adherence in canine peripheral veins. The complement-activating surfaces (NVP and VOH) cause preferential adhesion of leukocytes and were more thrombogenic than the low complement-activating surfaces (AAm, PE, and HEMA). Infusion of naja haje cobra venom factor depressed leukocyte adhesion, followed by a marked decrease in thrombogenesis, for the strong classical-pathway-activating surface (NVP). Although estimation of in vitro activation for AC was inconclusive because of a large effect of adsorption, AC behaved like VOH in vivo. These results suggest that C5a(des Arg) mediated activation of leukocytes may play a role in thrombus formation by complement activation on polymer surfaces.
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Authors | K Hayashi, H Fukumura, N Yamamoto |
Journal | Journal of biomedical materials research
(J Biomed Mater Res)
Vol. 24
Issue 10
Pg. 1385-95
(Oct 1990)
ISSN: 0021-9304 [Print] United States |
PMID | 2283355
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Complement Activation
(drug effects)
- Dogs
- In Vitro Techniques
- Microscopy, Electron, Scanning
- Platelet Adhesiveness
- Polyethylenes
(chemistry, pharmacology)
- Surface Properties
- Thrombosis
(etiology)
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