Abstract | PURPOSE: EXPERIMENTAL DESIGN: Archival HNSCC tissues including 99 nonmetastatic primary tumors and 101 metastatic primary tumors were examined for the association of integrin β1 expression with metastasis and disease prognosis by appropriate statistical methods. Fluorescence-activated cell sorting was used to separate the integrin β1(high/+) cell population from the integrin β1(low/-) population in HNSCC cell lines. These two populations and integrin β1 shRNA knockdown HNSCC cells were examined for the effect of integrin β1 on invasion in vitro and on lymph node and lung metastases in a xenograft mouse model. Expression and activation of matrix metalloproteinases ( MMP) were examined by zymography. RESULTS: Statistical analysis showed that integrin β1 expression was significantly higher in the metastatic primary tumors than in the nonmetastatic tumors (42.6% vs. 24.8%, P < 0.0001 and P < 0.0001 by univariate and multivariate analyses, respectively). In patients with lymph node metastasis, integrin β1 expression was inversely correlated with overall survival (P = 0.035). The integrin β1 knockdown or integrin β1(low/-) HNSCC cells showed a significant reduction in lymph node and lung metastases in vivo (P < 0.001 and P < 0.05, respectively). Significantly reduced Matrigel invasion capability was also found in integrin β1 knockdown or integrin β1(low/-) HNSCC cells (P < 0.01). Finally, zymography results showed integrin β1-affected HNSCC invasion by regulating MMP-2 activation. CONCLUSION: These findings indicate that integrin β1 has a major impact on HNSCC prognosis through its regulation of metastasis.
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Authors | Dongsheng Wang, Susan Müller, A R M Ruhul Amin, Donghai Huang, Ling Su, Zhongliang Hu, Mohammad Aminur Rahman, Sreenivas Nannapaneni, Lydia Koenig, Zhengjia Chen, Mourad Tighiouart, Dong M Shin, Zhuo G Chen |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 18
Issue 17
Pg. 4589-99
(Sep 01 2012)
ISSN: 1557-3265 [Electronic] United States |
PMID | 22829201
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Integrin beta1
- RNA, Small Interfering
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Animals
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Disease-Free Survival
- Female
- Flow Cytometry
- Follow-Up Studies
- Gene Expression Regulation, Neoplastic
- Head and Neck Neoplasms
(metabolism, pathology)
- Humans
- Integrin beta1
(genetics, metabolism)
- Lymphatic Metastasis
(pathology)
- Male
- Mice
- Middle Aged
- Neoplasm Invasiveness
- Neoplasm Staging
- Prognosis
- RNA, Small Interfering
- Transplantation, Heterologous
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