Gold nanoparticle based dot-blot immunoassay for sensitively detecting Alzheimer's disease related β-amyloid peptide.

Abstract

A simple, sensitive gold nanoparticle (GNP)-based dot-blot immunoassay has been developed for detecting Alzheimer's disease related β-amyloid peptide 1-42 (Aβ(1-42)) down to a 50 pg mL(-1) level in aqueous solution. Practical samples (cerebrospinal fluid (CSF), cell culturing mediums and cell lysates) have been used to demonstrate the ability of the assay.

• Authors: Chengke Wang, Dianjun Liu, Zhenxin Wang
• Journal: Chemical communications (Cambridge, England) (Chem Commun (Camb)) Vol. 48 Issue 67 Pg. 8392-4 (Aug 28 2012) ISSN: 1364-548X [Electronic] England
• PMID: 22796866 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid beta-Peptides
• Gold

Topics

• Alzheimer Disease (diagnosis)
• Amyloid beta-Peptides (chemistry)
• Cell Line
• Gold (chemistry)
• Humans
• Immunoassay
• Metal Nanoparticles (chemistry)
• Models, Molecular
• Sensitivity and Specificity