Abstract |
Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M ( IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4% (95% confidence interval [CI] = 51-78) (Xcyton), 69.2% (95% CI = 55-81) (Panbio), however 96.2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively.
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Authors | Catrin E Moore, Stuart D Blacksell, Thaksinaporn Taojaikong, Richard G Jarman, Robert V Gibbons, Sue J Lee, Vilada Chansamouth, Soulignasack Thongpaseuth, Mayfong Mayxay, Paul N Newton |
Journal | The American journal of tropical medicine and hygiene
(Am J Trop Med Hyg)
Vol. 87
Issue 1
Pg. 171-178
(Jul 2012)
ISSN: 1476-1645 [Electronic] United States |
PMID | 22764310
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Central Nervous System Infections
(blood, cerebrospinal fluid, diagnosis)
- Child
- Encephalitis, Japanese
(blood, cerebrospinal fluid, diagnosis)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Immunoglobulin M
(immunology)
- Laos
- Male
- Prospective Studies
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