Production and characterization of scFvA33T1, an immunoRNase targeting colon cancer cells.

Abstract

Within the last 10 years, the use of different RNases as therapeutic agents for various diseases has been pursued. Furthermore, the advancements of recombinant technology have allowed the assembly of proteins with different functions. In this regard, immunoribonucleases (immunoRNases) stand out as some of the most promising therapeutic candidates given their enzymatic and non-mutagenic character. Accordingly, the work reported here describes fusing RNase T1, one of the most studied members of the microbial RNase family, to the single-chain variable fragment (scFv) of a monoclonal antibody that targets the glycoprotein A33 antigen (GPA33) from human colon cancer cells. A heterologous production system, which employs the yeast Pichia pastoris, has been optimized to produce this immunoRNase (scFvA33T1) with yields of ∼ 5-10 mg · L(-1). The purified protein appears to be correctly folded as it retains its antigen specificity and ribonucleolytic activity. Finally, it also shows specific binding to, internalization into and toxicity against GPA33-positive cell lines compared with the control, GPA33-negative cells. Overall, it can be concluded that scFvA33T1 is a promising therapeutic fusion protein with the additional advantage that presumably it can be produced and purified in large amounts using an easily scalable yeast-based system.

• Authors: Jaime Tomé-Amat, Aida Menéndez-Méndez, Lucía García-Ortega, Carl A Batt, Mercedes Oñaderra, Alvaro Martínez-del-Pozo, José G Gavilanes, Javier Lacadena
• Journal: The FEBS journal (FEBS J) Vol. 279 Issue 17 Pg. 3022-32 (Sep 2012) ISSN: 1742-4658 [Electronic] England
• PMID: 22748038 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 The Authors Journal compilation © 2012 FEBS.

Chemical References

• Single-Chain Antibodies
• Ribonuclease T1

Topics

• Circular Dichroism
• Colonic Neoplasms (enzymology, immunology, pathology)
• Electrophoresis, Polyacrylamide Gel
• Endocytosis
• Humans
• Ribonuclease T1 (metabolism)
• Single-Chain Antibodies
• Spectrophotometry, Ultraviolet