Downregulation of fetuin-B and zinc-α2-glycoprotein is linked to impaired fatty acid metabolism in liver cells.

Abstract

BACKGROUND:

Our recent proteomic study has shown that plasma protein levels of fetuin-B (Ft-B) and zinc-α2-glycoprotein (ZAG) are significantly elevated in obesity-resistant (OR) rats exposed to a high fat diet. Time profiling of the plasma concentrations of Ft-B and ZAG in OR rats has shown stable regulation of these proteins throughout the entire period of rat breeding.

METHODS:

To firmly establish roles for these proteins in lipogenesis, we efficiently knocked down (KD) the genes FETUB and AZGP1 encoding Ft-B and ZAG, respectively, using siRNA in Chang liver cells.

RESULTS:

Reduced expression of FETUB and AZGP1 led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels in both KD cells. Collectively with our previous findings, we confirmed that Ft-B was similarly regulated with Ft-A, in that their plasma protein levels were commonly reduced in diet-induced obese rats.

CONCLUSION:

Our results provide a possible relationship between reduced plasma protein levels of Ft-B and ZAG and higher risk of diet-induced obesity through impaired fatty acid metabolism in hepatocytes.

• Authors: Jung-Won Choi, Hao Liu, Rajib Mukherjee, Jong Won Yun
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 30 Issue 2 Pg. 295-306 ( 2012) ISSN: 1421-9778 [Electronic] Germany
• PMID: 22739111 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 S. Karger AG, Basel.

Chemical References

• Fatty Acids
• Fetuin-B
• RNA, Small Interfering
• Seminal Plasma Proteins
• Zn-Alpha-2-Glycoprotein

Topics

• Animals
• Cell Line
• Diet, High-Fat
• Down-Regulation
• Electrophoresis, Gel, Two-Dimensional
• Fatty Acids (metabolism)
• Fetuin-B (antagonists & inhibitors, genetics, metabolism)
• Hepatocytes (metabolism)
• Lipogenesis
• Male
• RNA Interference
• RNA, Small Interfering (metabolism)
• Rats
• Rats, Sprague-Dawley
• Seminal Plasma Proteins (antagonists & inhibitors, genetics, metabolism)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Zn-Alpha-2-Glycoprotein