Immunization of mice by hollow mesoporous silica nanoparticles as carriers of porcine circovirus type 2 ORF2 protein.

Abstract

BACKGROUND:

Porcine circovirus type 2 (PCV2) is a primary etiological agent of post-weaning multi-systemic wasting syndrome (PMWS), which is a disease of increasing importance to the pig industry worldwide. Hollow mesoporous silica nanoparticles (HMSNs) have gained increasing interest for use in vaccines.

METHODS:

To study the potential of HMSNs for use as a protein delivery system or vaccine carriers. HMSNs were synthesized by a sol-gel/emulsion(oil-in-water/ethanol) method, purified PCV2 GST-ORF2-E protein was loaded into HMSNs, and the resulting HMSN/protein mixture was injected into mice. The uptake and release profiles of protein by HMSNs in vitro were investigated. PCV2 GST-ORF2-E specific antibodies and secretion of IFN-γ were detected by enzyme-linked immunosorbent assays, spleen lymphocyte proliferation was measured by the MTS method, and the percentage of CD4+ and CD8+ were determined by flow cytometry.

RESULTS:

HMSNs were found to yield better binding capacities and delivery profiles of proteins; the specific immune response induced by PCV2 GST-ORF2-E was maintained for a relatively long period of time after immunization with the HMSN/protein complex.

CONCLUSION:

The findings suggest that HMSNs are good protein carriers and have high potential for use in future applications in therapeutic drug delivery.

• Authors: Hui-Chen Guo, Xiao-Ming Feng, Shi-Qi Sun, Yan-Quan Wei, De-Hui Sun, Xiang-Tao Liu, Zai-Xin Liu, Jian-Xiong Luo, Hong Yin
• Journal: Virology journal (Virol J) Vol. 9 Pg. 108 (Jun 12 2012) ISSN: 1743-422X [Electronic] England
• PMID: 22691538 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Drug Carriers
• Viral Proteins
• Viral Vaccines
• Silicon Dioxide
• Interferon-gamma

Topics

• Animals
• Antibodies, Viral (blood)
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Cell Proliferation
• Circovirus (immunology)
• Drug Carriers (administration & dosage)
• Interferon-gamma (metabolism)
• Mice
• Nanoparticles (administration & dosage)
• Silicon Dioxide (administration & dosage)
• Spleen (immunology)
• Vaccination (methods)
• Viral Proteins (immunology)
• Viral Vaccines (administration & dosage, immunology)