Abstract |
The present study characterized the in vitro biological response of a comprehensive set of cancer cell lines to gold nanoparticles (2.7 nm) coated with tiopronin (AuNPs-TP). Our findings suggest that upon entering cells, the AuNPs-TP are sequestered in vacuoles such as endosomes and lysosomes, and mostly localize in perinuclear areas. Peak cell accumulation was achieved at 8 hours after incubation. L929 and H520 cells showed more than 75% surviving fraction when treated with 0.5 mg/mL of AuNPs-TP for 24 hours, whereas the surviving fractions were 60% in MCF-7 and 20% in HeLa cells. Reactive oxygen species (ROS) production by the AuNPs-TP was dependent on cell line and exposure time. Antioxidants inhibited ROS generation to various extents, with glutathione and tiopronin being most effective. Overall, exposure time, concentration of the AuNPs-TP, and cell line influenced neoplastic cell response. Furthermore, the mechanism of cytotoxicity of the AuNPs-TP was found to be ROS generation. FROM THE CLINICAL EDITOR: This study describes the basic intracellular characteristics of Tiopronin-Au nanoparticles from the standpoint of their anti- cancer activity in different cancer cell cultures.
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Authors | Lei Cui, Payam Zahedi, Justin Saraceno, Robert Bristow, David Jaffray, Christine Allen |
Journal | Nanomedicine : nanotechnology, biology, and medicine
(Nanomedicine)
Vol. 9
Issue 2
Pg. 264-73
(Feb 2013)
ISSN: 1549-9642 [Electronic] United States |
PMID | 22687897
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- Reactive Oxygen Species
- Gold
- Tiopronin
- Glutathione
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Topics |
- Animals
- Antioxidants
(metabolism)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Glutathione
(metabolism)
- Gold
(chemistry, pharmacokinetics, pharmacology)
- Humans
- Mice
- Nanoparticles
(chemistry)
- Neoplasms
(drug therapy, metabolism)
- Reactive Oxygen Species
(metabolism)
- Tiopronin
(chemistry, pharmacokinetics, pharmacology)
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