Continuous regional arterial infusion with fluorouracil and octreotide attenuates severe acute pancreatitis in a canine model.

Abstract

AIM:

To investigate the therapeutic effects of fluorouracil (5-Fu) and octreotide (Oct) continuous regional arterial infusion (CRAI,) alone or in combination, was administered in a canine model of severe acute pancreatitis (SAP).

MATERIALS AND METHODS:

The animals were divided into five groups; group A (Sham), group B (SAP), group C (SAP and 5-Fu), group D (SAP and Oct), and group E (SAP and 5-Fu + Oct). Levels of amylase, α-tumor necrosis factor (TNF-α), blood urea nitrogen (BUN), creatinine, thromboxane B2 and 6-keto- prostaglandin F1α were measured both before and after the induction of SAP. Pathologic examination of the pancreas and kidneys was performed after termination of the study.

RESULTS:

Pathologic changes noted in the pancreas in SAP significantly improved following CRAI with either single or combined administration of 5-Fu and Oct, where combination therapy demonstrated the lowest injury score. All treatment groups had significantly lower levels of serum TNF-α and amylase activity (P<0.05), though only groups D and E had a lower BUN level as compared to group B. The plasma thromboxane B(2) level increased in SAP, but the ratio of thromboxane B(2)/6-keto- prostaglandin F(1α) decreased in the treatment groups, with the combination therapy (group E) demonstrating the lowest ratio as compared to the other 3 experimental groups (P<0.05).

CONCLUSIONS:

The findings in the present study demonstrate an attenuation of SAP in a canine model following CRAI administration with 5-Fu or Oct, alone or in combination.

• Authors: Meng Tao Zhou, Bi Cheng Chen, Hong Wei Sun, Yue Peng Jin, Fa Jing Yang, Xing Zhang, Roland Andersson, Qi Yu Zhang
• Journal: PloS one (PLoS One) Vol. 7 Issue 5 Pg. e37347 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 22655040 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimetabolites
• Gastrointestinal Agents
• Tumor Necrosis Factor-alpha
• Thromboxane B2
• Creatinine
• Amylases
• Octreotide
• Fluorouracil

Topics

• Amylases (blood)
• Animals
• Antimetabolites (administration & dosage, therapeutic use)
• Creatinine (blood)
• Dogs
• Female
• Fluorouracil (administration & dosage, therapeutic use)
• Gastrointestinal Agents (administration & dosage, therapeutic use)
• Infusions, Intra-Arterial
• Kidney (drug effects, pathology)
• Male
• Octreotide (administration & dosage, therapeutic use)
• Pancreas (drug effects, pathology)
• Pancreatitis (drug therapy, pathology)
• Thromboxane B2 (blood)
• Tumor Necrosis Factor-alpha (blood)