Abstract | PURPOSE: MATERIALS AND METHODS: 17 CD24 knockout (KO) and 12 wild-type (WT) C57BL/6 mice were used. Group 1 mice were exposed to oxygen concentrations of 75 ± 2% from postnatal day (P) 7 to P12. Group 2 mice were raised in room air. At P17, all mice underwent fluorescein-conjugated- dextran perfusion and were sacrificed. The flat-mounted retinas were scored manually and digitally by a new computerized algorithm, according to blood vessel obliteration, tortuosity, vascular tufts and neovascularization formation. RESULTS: Fifty four retinal whole mounts were available for analysis and scoring. Group 1 retinas had significantly higher values of vaso-obliteration, tufts, neovascularization, vessel tortuosity and higher mean retinopathy scores than Group 2 retinas (KO mice: 9.0 ± 0.27 vs. 0.74 ± 0.2, respectively, P < 0.0001; WT mice: 7.58 ± 0.40 vs. 1.17 ± 0.27, respectively, P < 0.0001). Manual scoring in Group 1 revealed higher values of neovascularization, tortuosity and mean retinopathy scores in KO mice vs. WT mice (9.0 ± 0.27 vs. 7.58 ± 0.40, respectively, P = 0.009). Digital scoring revealed a higher neovascularization score in KO mice as well (13.72 ± 0.82% vs. 8.06 ± 0.27%, P < 0.0001). All mice had similar vaso-obliteration areas. There were no significant differences between KO and WT mice in Group 2. CONCLUSIONS: Absence of CD24 may have a deleterious effect on angiogenesis occurring in the second stage of ROP development, though its role in vessel obliteration during the first stage of ROP is probably limited.
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Authors | Hadas Newman, Shiran Shapira, Oriel Spierer, Sarah Kraus, Mordechai Rosner, Sarah Pri-Chen, Anat Loewenstein, Nadir Arber, Adiel Barak |
Journal | Current eye research
(Curr Eye Res)
Vol. 37
Issue 6
Pg. 532-9
(Jun 2012)
ISSN: 1460-2202 [Electronic] England |
PMID | 22577772
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD24 Antigen
- Cd24a protein, mouse
- Dextrans
- Fluoresceins
- fluorescein-dextran
- Oxygen
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Topics |
- Animals
- Animals, Newborn
- CD24 Antigen
(physiology)
- Dextrans
(metabolism)
- Disease Models, Animal
- Fluoresceins
(metabolism)
- Humans
- Hyperoxia
(metabolism, physiopathology)
- Infant, Newborn
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Oxygen
(toxicity)
- Retinal Neovascularization
(metabolism, physiopathology)
- Retinal Vessels
(pathology)
- Retinopathy of Prematurity
(metabolism, physiopathology)
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