Use of a cell hybrid test system to demonstrate that benomyl induces aneuploidy and polyploidy.

Abstract

We have monitored the segregation of a single human chromosome in a human-Chinese hamster hybrid cell line, EUBI, following exposure to benomyl. We found a dose-dependent increase in frequency of aneuploidy, but a much more marked induction of polyploidy was noted at the highest benomyl concentration. We confirm the usefulness of this assay for determining genetic risk associated with human exposure to environmental chemicals.

• Authors: P A Zelesco, I Barbieri, J A Graves
• Journal: Mutation research (Mutat Res) Vol. 242 Issue 4 Pg. 329-35 (Dec 1990) ISSN: 0027-5107 [Print] Netherlands
• PMID: 2255326 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benomyl

Topics

• Aneuploidy
• Animals
• Benomyl (toxicity)
• Cell Line
• Chromosomes (drug effects)
• Cricetinae
• Cricetulus
• Humans
• Hybrid Cells (drug effects)
• Karyotyping
• Mutagenicity Tests (methods)
• Polyploidy