Abstract | BACKGROUND: METHODS: The present study was to investigate the effect of PESV on cell proliferation, cell cycle, and apoptosis in human androgen-independent prostate cancer cells DU-145 in vitro. RESULTS: PESV treatment on these cells resulted in a significantly dose-dependent growth inhibition with a G1 phase arrest at 40μg/mL after 48h treatment. PESV treatment strongly induced expression of p27 (Kip1), but resulted in a decrease in cyclin E, one of cyclins involved in G1 progression. In other studies, PESV treatment also induced high apoptosis index (AI), confirmed by TdTmediated dUTP- biotin nick-end labeling (TUNEL) assay. Further, the apoptosis induction by PESV (40μg/mL) in DU145 cells was associated with an increase of pro-apoptotic protein Bax. CONCLUSIONS: These results suggest that PESV modulates the expression of cell cycle-related and apoptosis-related proteins and induces growth inhibition and apoptosis of DU145 cells, providing a strong rationale for future studies to evaluate prevention or/and intervention strategies for PESV in pre-clinical prostate cancer models. KEYWORDS:
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Authors | Yue Ying Zhang, Li Cun Wu, Zhao Peng Wang, Zhao Xia Wang, Qing Jia, Guo Sheng Jiang, Wei Dong Zhang |
Journal | Journal of clinical medicine research
(J Clin Med Res)
Vol. 1
Issue 1
Pg. 24-31
(Apr 2009)
ISSN: 1918-3003 [Print] Canada |
PMID | 22505961
(Publication Type: Journal Article)
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