Abstract | BACKGROUND: METHODS: We describe the analysis of C26:0-LPC in dried-blood spots (DBS) using a rapid (30 min) and simple extraction procedure, isocratic HPLC resolution of LPC, and structure-specific analysis via negative ion mode tandem mass spectrometry. RESULTS: In putative normal DBS specimens from newborns (N=223) C26:0-LPC was 0.09±0.03 μmol/l whole blood, while in peroxisomal biogenesis disorder (including X-ALD) patients (N=28) C26:0-LPC was 1.13±0.67 μmol/l whole blood. Both multiple reaction monitoring and a neutral loss scan (225.1 Da) analysis of DBS were used to analyze LPC. CONCLUSIONS: Compared to a previous report of C26:0-LPC analysis in DBS, the method described here is simpler, faster, and more structure-specific for LPC with C26:0 acyl chains.
|
Authors | Christopher A Haynes, Víctor R De Jesús |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 413
Issue 15-16
Pg. 1217-21
(Aug 16 2012)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 22503909
(Publication Type: Evaluation Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Copyright | Published by Elsevier B.V. |
Chemical References |
|
Topics |
- Adrenoleukodystrophy
(blood, diagnosis, genetics)
- Chromatography, High Pressure Liquid
(methods)
- Dried Blood Spot Testing
(methods)
- Humans
- Infant, Newborn
- Lysophosphatidylcholines
(blood)
- Neonatal Screening
- Peroxisomal Disorders
(blood)
- Reference Values
- Spectrometry, Mass, Electrospray Ionization
(methods)
- Tandem Mass Spectrometry
|