GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning: a preliminary study.

Abstract

Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.

• Authors: Kenny F Lin, Hank H Sun, Matthew R Macewan, Susan E Mackinnon, Philip J Johnson
• Journal: Microsurgery (Microsurgery) Vol. 32 Issue 5 Pg. 370-6 (Jul 2012) ISSN: 1098-2752 [Electronic] United States
• PMID: 22473739 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Wiley-Liss, Inc.

Chemical References

• Gdnf protein, mouse
• Glial Cell Line-Derived Neurotrophic Factor
• Neuromuscular Agents
• Botulinum Toxins, Type A

Topics

• Animals
• Botulinum Toxins, Type A (administration & dosage, pharmacology)
• Glial Cell Line-Derived Neurotrophic Factor (metabolism, physiology)
• Mice
• Mice, Transgenic
• Muscle Strength (drug effects, physiology)
• Muscle, Skeletal (drug effects, metabolism, pathology, physiology)
• Muscular Atrophy (chemically induced)
• Nerve Block (methods)
• Neuromuscular Agents (administration & dosage, pharmacology)
• Organ Size
• Recovery of Function (physiology)
• Up-Regulation