Abstract | CONTEXT: OBJECTIVE: We studied circulating miRNA profiles in patients with PTC or benign nodules and healthy controls to identify serum miRNA that may be useful as markers for PTC. DESIGN, SETTING, AND PARTICIPANTS: Genome-wide serum miRNA expression profiles were determined using Solexa sequencing followed by extensive quantitative RT-PCR validation in 245 subjects (106 patients with PTC, 95 patients with benign nodules, and 44 healthy controls). A panel of miRNA was used to assess the expression of specific miRNA in the sera and thyroid tissues of patients with PTC or benign nodules. RESULTS: The expression of serum let-7e, miR-151-5p, and miR-222 was significantly increased in PTC cases relative to benign cases and healthy controls. Receiver operating characteristic curve analyses indicated that use of these three miRNA had a high diagnostic sensitivity and specificity for PTC. Serum let-7e, miR-151-5p, and miR-222 levels were found to be well correlated with certain clinicopathological variables, such as nodal status, tumor size, multifocal lesion status, and Tumor-Node- Metastasis stage. Expression of serum miR-151-5p and miR-222 in a subset of PTC patients decreased significantly after tumor excision. Increased expression of miR-151-5p and miR-222 was also found in the tissue of PTC patients. CONCLUSIONS: Our study demonstrates that serum miRNA profiles may be used as novel and minimally invasive diagnostic markers for PTC.
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Authors | Shuang Yu, Yuanyuan Liu, Jingsong Wang, Zhuming Guo, Quan Zhang, Fengyan Yu, Yunjian Zhang, Kai Huang, Yanbing Li, Erwei Song, Xi-long Zheng, Haipeng Xiao |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 97
Issue 6
Pg. 2084-92
(Jun 2012)
ISSN: 1945-7197 [Electronic] United States |
PMID | 22472564
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- MicroRNAs
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Topics |
- Adult
- Biomarkers, Tumor
(blood)
- Carcinoma, Papillary
(blood, diagnosis, genetics)
- Diagnosis, Differential
- Female
- Humans
- Male
- MicroRNAs
(blood)
- Middle Aged
- Neoplasms
(blood, diagnosis, genetics)
- Predictive Value of Tests
- Real-Time Polymerase Chain Reaction
- Sensitivity and Specificity
- Thyroid Neoplasms
(blood, diagnosis, genetics)
- Thyroid Nodule
(blood, diagnosis, genetics)
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