Silver nanoparticles modify VEGF signaling pathway and mucus hypersecretion in allergic airway inflammation.

Abstract

The anti-inflammatory action of silver nanoparticles (NPs) has been reported in a murine model of asthma in a previous study. But more specific mechanisms of silver NPs in an attenuation of allergic airway inflammation have not yet been established. Vascular and mucous changes are believed to contribute largely in pathophysiology in asthma. Among various factors related to vascular changes, vascular endothelial growth factor (VEGF) plays a pivotal role in vascular changes in asthma. Mucin proteins MUC5AC and MUC5B have been implicated as markers of goblet cell metaplasia in lung pathologies. The aim of this study was to investigate the effects of silver NPs on VEGF signaling pathways and mucus hypersecretion. Ovalbumin (OVA)-inhaled female BALBc mice were used to evaluate the role of silver NPs and the related molecular mechanisms in allergic airway disease. In this study, with an OVA-induced murine model of allergic airway disease, it was found that the increased levels of hypoxia-inducible factor (HIF)-1α, VEGF, phosphatidylinositol-3 kinase (PI3K) and phosphorylated-Akt levels, and mucous glycoprotein expression (Muc5ac) in lung tissues were substantially decreased by the administration of silver NPs. In summary, silver NPs substantially suppressed mucus hypersecretion and PI3K/HIF-1α/VEGF signaling pathway in an allergic airway inflammation.

• Authors: Sunhyae Jang, Ji Won Park, Hye Rim Cha, Sun Young Jung, Jeong Eun Lee, Sung Soo Jung, Ju Ock Kim, Sun Young Kim, Choong Sik Lee, Hee Sun Park
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 7 Pg. 1329-43 ( 2012) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 22457593 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hypoxia-Inducible Factor 1, alpha Subunit
• Interleukins
• Muc5ac protein, mouse
• Mucin 5AC
• Vascular Endothelial Growth Factor A
• Silver

Topics

• Animals
• Bronchi (drug effects, pathology)
• Bronchoalveolar Lavage Fluid (cytology)
• Dose-Response Relationship, Drug
• Female
• Histocytochemistry
• Human Umbilical Vein Endothelial Cells
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Interleukins (metabolism)
• Lung (drug effects, metabolism)
• Metal Nanoparticles (chemistry)
• Mice
• Mice, Inbred BALB C
• Mucin 5AC (metabolism)
• Mucus (metabolism)
• Pneumonia (chemically induced, metabolism, physiopathology)
• Signal Transduction (drug effects)
• Silver (chemistry, pharmacology)
• Vascular Endothelial Growth Factor A (metabolism)