Abstract |
To assess the potency of regulatory T (Treg) cells induced against an irrelevant Ag, mice were orally vaccinated with Salmonella expressing Escherichia coli colonization factor antigen I fimbriae. Isolated CD25⁺ and CD25⁻CD4⁺ T cells were adoptively transferred to naive mice, and Treg cells effectively protected against experimental autoimmune encephalomyelitis (EAE), unlike Treg cells from Salmonella vector-immunized mice. This protection was abrogated upon in vivo neutralization of TGF-β, resulting in elevated IL-17 and loss of IL-4 and IL-10 production. Thus, Treg cells induced to irrelevant Ags offer a novel approach to treat autoimmune diseases independent of auto-Ag.
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Authors | Sangmu Jun, Javier Ochoa-Repáraz, Dagmara Zlotkowska, Teri Hoyt, David W Pascual |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 245
Issue 1-2
Pg. 39-47
(Apr 2012)
ISSN: 1872-8421 [Electronic] Netherlands |
PMID | 22418032
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- Epitopes, T-Lymphocyte
- Interleukin-17
- Myelin Proteolipid Protein
- Plp1 protein, mouse
- Transforming Growth Factor beta
- Interleukin-10
- Interleukin-4
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Topics |
- Adoptive Transfer
(methods)
- Animals
- Bystander Effect
(immunology)
- Disease Models, Animal
- Down-Regulation
(immunology)
- Encephalomyelitis, Autoimmune, Experimental
(immunology, pathology, therapy)
- Epitopes, T-Lymphocyte
(immunology)
- Female
- Interleukin-10
(antagonists & inhibitors, biosynthesis)
- Interleukin-17
(biosynthesis, physiology)
- Interleukin-4
(antagonists & inhibitors, biosynthesis)
- Mice
- Mice, Inbred Strains
- Myelin Proteolipid Protein
(immunology)
- Primary Cell Culture
- T-Lymphocytes, Regulatory
(immunology, metabolism, transplantation)
- Transforming Growth Factor beta
(antagonists & inhibitors, physiology)
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