[Isoenzyme spectrum and kinetic properties of pyruvate kinase from the liver of thiamine-deficient rats].

Abstract

Thiamine-deficiency in animals induced by everyday subcutaneous administration of oxythiamine in a dose of 4, 40 and 100 mg/kg of weight for 10 days results in a decrease of the total activity of pyruvate kinase in the liver tissue and does not affect the mentioned index in the kidney and heart tissues. It is shown that as a result of the enzyme fractionation in the column with DEAE-cellulose the total activity of pyruvate kinase in the liver tissue of rats with thiamine-deficiency decreases due to L-isoform while the content of M-isoform remains unchanged. Thiamine deficiency does not affect kinetic characteristics of the L-isoform, extracted from the liver and this shows the absence of changes in the degree of phosphorylation of pyruvate kinase L-isoform under these conditions.

• Authors: O V Konovalenko, S S Maglysh, Z V Gorbach
• Journal: Ukrainskii biokhimicheskii zhurnal (1978) (Ukr Biokhim Zh (1978)) 1990 Jul-Aug Vol. 62 Issue 4 Pg. 50-5 ISSN: 0201-8470 [Print] Ukraine
• Vernacular Title: Izofermentnyĭ spektr i kineticheskie svoĭstva piruvatkinazy pecheni tiamindefitsitnykh krys.
• PMID: 2238154 (Publication Type: English Abstract, Journal Article)

Chemical References

• Isoenzymes
• Pyruvate Kinase

Topics

• Animals
• Chromatography, DEAE-Cellulose
• Isoenzymes (antagonists & inhibitors, metabolism)
• Kidney (enzymology)
• Kinetics
• Liver (enzymology)
• Male
• Myocardium (enzymology)
• Organ Specificity
• Pyruvate Kinase (antagonists & inhibitors, metabolism)
• Rats
• Thiamine Deficiency (enzymology)