Glycomics for drug discovery: metabolic perturbation in androgen-independent prostate cancer cells induced by unnatural hexosamine mimics.

Abstract

Inhibited: N-acetylglucosamine (GlcNAc) derivatives with a fluorine atom at the C4 position (2-4) were synthesized, and their ability to inhibit cancer-cell growth was investigated. The administration of these 4F-GlcNAc derivatives to cells led to the unnatural sugar nucleotide 1. Furthermore, N-glycan profiles of cells were determined by using a glycoblotting-based enrichment analysis, which is suitable for high-throughput screenings for drug discovery.

• Authors: Shin-Ichiro Nishimura, Megumi Hato, Satoshi Hyugaji, Fei Feng, Maho Amano
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 51 Issue 14 Pg. 3386-90 (Apr 02 2012) ISSN: 1521-3773 [Electronic] Germany
• PMID: 22345046 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Androgens
• Fluorine
• Acetylglucosamine

Topics

• Acetylglucosamine (chemical synthesis, chemistry, toxicity)
• Androgens (metabolism)
• Apoptosis (drug effects)
• Carbohydrate Sequence
• Cell Line, Tumor
• Drug Evaluation, Preclinical
• Fluorine (chemistry)
• Glycomics
• Glycosylation
• Humans
• Male
• Molecular Sequence Data
• Prostatic Neoplasms (metabolism, pathology)