Platelet-rich plasma inhibits the apoptosis of highly adipogenic homogeneous preadipocytes in an in vitro culture system.

Abstract

Auto-transplantation of adipose tissue is commonly used for the treatment of tissue defects in plastic surgery. The survival of the transplanted adipose tissue is not always constant, and one of reasons is the accelerated apoptosis of the implanted preadipocytes. We have recently established highly homogeneous preadipocytes, named ccdPAs. The aim of the current study was to evaluate the regulation of the potency of platelet-rich plasma (PRP) on the apoptosis of ccdPAs in vitro. PRP stimulated the proliferation of the preadipocytes in a dose-dependent manner, and the stimulatory activity of 2% PRP was significantly higher than that of 2% FBS or 2% platelet-poor plasma (PPP). The presence of 2% PRP significantly inhibited serum starvation- or TNF-α/cycloheximide-induced apoptosis in comparison to 2% FBS or 2% PPP. DAPK1 and Bcl-2-interacting mediator of cell death (BIM) mRNAs were reduced in the preadipocytes cultured with 2% PRP in comparison to those cultured in 2% FBS. The gene expression levels were significantly higher in cells cultured without serum in comparison to cells cultured with 2% FBS, and the levels in the cells with 2% PRP were reduced to 5-10% of those in the cells without serum. These results indicated that ccdPAs exhibit anti- apoptotic activities, in addition to increased proliferation, when cultured in 2% PRP in comparison to the same concentration of FBS, and that this was accompanied with reduced levels of DAPK1 and BIM mRNA expression in in vitro culture. PRP may improve the outcome of transplantation of adipose tissue by enhancing the anti-apoptotic activities of the implanted preadipocytes.

• Authors: Yoshitaka Fukaya, Masayuki Kuroda, Yasuyuki Aoyagi, Sakiyo Asada, Yoshitaka Kubota, Yoshitaka Okamoto, Toshinori Nakayama, Yasushi Saito, Kaneshige Satoh, Hideaki Bujo
• Journal: Experimental & molecular medicine (Exp Mol Med) Vol. 44 Issue 5 Pg. 330-9 (May 31 2012) ISSN: 2092-6413 [Electronic] United States
• PMID: 22314197 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Regulatory Proteins
• BCL2L11 protein, human
• Bcl-2-Like Protein 11
• Membrane Proteins
• Proto-Oncogene Proteins
• DAPK1 protein, human
• Death-Associated Protein Kinases
• Calcium-Calmodulin-Dependent Protein Kinases

Topics

• Adipocytes (cytology)
• Adipose Tissue (cytology, metabolism)
• Apoptosis (physiology)
• Apoptosis Regulatory Proteins (antagonists & inhibitors, metabolism)
• Bcl-2-Like Protein 11
• Calcium-Calmodulin-Dependent Protein Kinases (antagonists & inhibitors, metabolism)
• Cell Culture Techniques (methods)
• Cell Differentiation
• Cell Proliferation
• Cells, Cultured
• Death-Associated Protein Kinases
• Gene Expression Regulation
• Humans
• Membrane Proteins (antagonists & inhibitors, metabolism)
• Platelet-Rich Plasma (metabolism, physiology)
• Proto-Oncogene Proteins (antagonists & inhibitors, metabolism)
• Tissue Transplantation